Posts Tagged: huanglongbing
VISALIA – Last week's California Citrus Conference marked a major milestone for growers, and it wasn't just the 50th anniversary of the Visalia-based Citrus Research Board (CRB). It was a resounding revelation that new research may cure the greatest threat to the citrus industry in the next few years.
Michelle Heck, PhD, told the crowd of citrus growers at the Wyndham Hotel on Oct. 10 that her team might only need that much time to inbreed a generation of Asian citrus psyllids that are incapable of transmitting the deadly tree disease known as huanglongbing (HLB). The disease has already destroyed China's citrus industry, decimated Florida and Texas growing regions and is currently killing the citrus industry in Brazil.
One grower commented, “China's been dealing with this for 100 years and Brazil for 14 years. We've had this for four to five years in California and we are already knocking on the door of nailing it. That's impressive!”
Heck, a molecular biologist with the USDA Agricultural Research Service, was the first to lead a team of scientists to study the proteins involved in the interaction of the pest, plant and pathogen. One of those proteins creates a blue color in the blood of some psyllids. Her research revealed that psyllids containing the blue protein are far less efficient at transmitting HLB to the plant than others. She then bred those psyllids and took their progency and raised them on orange jasmine hedges, better known as Murraya, a plant the psyllids are attracted but is HLB resistant. The combination of the pest and plant reduced transmission of HLB to healthy citrus leaves from 32% to 2.9%.
Heck said the next steps are to continue breeding the pests that are poor transmitters of the disease to create a line of psyllids that do not transmit HLB at all. She said it would take another two years to breed an “optimized line” of the psyllid but once that was complete, that line could begin mass breeding for release.
“By sheer numbers, we can tip the scales [in the fight against HLB],” she said, “but it's unknown if these lines will out compete other psyllids [in the field].”
One grower asked if the non-transmitting line of the pest would be considered a genetically modified organism, or GMO, a distinction that could hurt fruit grown in groves with the new pest. Heck said all of the psyllids would be bred natuarally, so there is no genetic alteration of the insect itself.
“This is something the anti-GMO groups should feel good about,” Heck said.
Best Case Scenario
Victoria Hornbaker, Statewide Citrus Program Manager for the California Department of Food and Agriculture (CDFA), called the current HLB situation in California a best case scenario. She said the Citrus Pest and Disease Prevention Program's (CPDPP) No. 1 priority is to quickly detect and remove diseased trees. Shortly after the discovery of the first HLB tree in 2012, California's myriad of citrus agencies worked together to quickly implement measures to control movement of fruit and nursery stock, monitor and suppress the ACP population, and begin working on ways to detect the disease and possibly cure it.
“Instead of all commercial groves being covered by a quarantine, we said we're going to quarantine the whole state,” Hornbaker said.
By limiting the movement of citrus in and out of different quarantine zones, there is less likelihood of transporting trees from an infected area to an uninfected area. If any infected trees are discovered, they are removed, destroyed and replaced with a healthy tree. There are many early detection techniques (EDTs) being studied throughout the country, including looking for patterns in leaves, chemicals produced by trees in response to HLB, and studying molecules of the bacteria causing the disease. A recent analysis of these EDTs showed that most are about 95% effective in identifying an infected tree, and that losing 5% of healthy trees is an acceptable loss compared to devastation caused by the disease spreading unchecked.
While early detection methods of ACP are still being perfected, the fight to control the spread of the psyllid is not. After research identified the microscopic parasitic wasp radiate terminaxia as the natural enemy of the psyllid, they began working to mass produce and release them. To date, more than 11 million wasps have bee released in citrus growing regions since 2013, the closest being in Kern County.
Reproduced from Sun Gazette:
The Citrus Research Board is arranging to bring specially trained dogs to the UC Lindcove Research and Extension Center to test their ability to sniff out the devastating citrus disease huanglongbing, reported Bob Rodriguez in the Fresno Bee.
CRB president Gary Schulz is working with the USDA, which is training dogs in Florida to identify trees with huanglongbing soon after the trees are infected. HLB has ravaged Florida's citrus industry. In California, the disease has been found about 800 Southern California backyard trees, but officials have so far managed to keep it out of the state's commercial orchards.
"The USDA has invested million of dollars in detector dogs and they have proven to be a credible diagnostic tool for early detection and screening trees," Schulz said.
HLB is spread by Asian citrus psyllids. Psyllids can pick up the the disease from infected trees and spread it to other trees as they feed. Symptoms may not show up in the tree until a year or two after it is infected. PCR (polymerase chain reaction) is the only way to positively identify huanglongbing infection in citrus. The process requires testing of many leaves or branches from the tree and may return a false negative if the samples selected for testing aren't infected, but other parts of the tree are.
Schulz said the HLB-detection dogs will start their California work in the southern part of the state before traveling north.
The city of Riverside pitched a white tent over the "Parent Navel" orange tree at the intersection of Arlington and Magnolia avenues last week to protect it from the threat of huanglongbing disease, reported Ryan Hagen the Riverside Press Enterprise.
“The Parent Navel is an iconic symbol of Riverside, as it represents the impact the citrus industry had on our economy,” Mayor Rusty Bailey said in a press release issued by the City of Riverside. “Riversiders hold this symbol of our citrus heritage very dear, so it is encouraging to see our parks personnel taking a proactive approach.”
The tree was one of two planted by Eliza Tibbets in 1873, when she received the seedless orange cultivars from Florida by mail. Tibbets cared for the trees and sold budwood to nurseries, which led to extensive plantings of nursery trees cloned from hers.
Huanglongbing disease made its appearance in Riverside last year in residential trees. Officials are working to prevent its spread by controlling Asian citrus psyllid, the insect that can move the disease from tree to tree. Meanwhile researchers are searching for a cure.
The Parent Navel's high value led UC Riverside researchers and city officials to construct the large white barrier.
"It's not beautiful," said Georgios Vidalakis, UC Cooperative Extension specialist and director of the citrus clonal protection program at UC Riverside. "It's obstructing the tree from public view, and we apologize for that. But the risk from not doing that is catastrophic."
navel Washington in Riverside
The Citrus Research and Development Foundation (CRDF), a $124 million state citrus-industry initiative, has invested nearly 90 percent of its funds in HLB research. CRDF asked the Academies to review its research portfolio and determine if its efforts have followed recommendations outlined in the Academies' 2010 report, which originally called for the organization's creation. The committee found that CRDF was responsive to several recommendations from the previous report, and along with other funders, has advanced our knowledge about the disease. However, HLB remains a serious danger to Florida's citrus industry, having progressed from an acute to a chronic disease throughout the state.
The report notes that significant barriers to progress toward an HLB solution still exist, among them the inability to culture the bacteria in the laboratory, the lack of advanced diagnostics for early disease detection, and the absence of standardized research methodology that would improve the comparability of results across studies. Resolution of any one of these issues would constitute a significant step, according to the report.
The committee recommended continuing support for both basic and applied research for short- and long-term research efforts. In the long run, HLB solutions would likely utilize new technology, such as gene modification and gene editing, focusing on targets that mediate molecular interactions among plant, bacteria, and the vector, the committee said. As interest in using genetic modification in research grows, CRDF should also consider funding research to assess stakeholder acceptance of the technology and expand efforts to educate growers, processors, and consumers to facilitate the eventual deployment of genetically modified citrus lines.
In the meantime, growers in the state will need short-term solutions for the industry to remain viable. The report recommends finding the best suite of strategies to control the disease in different environmental and growing conditions, vector and pathogen pressures, tree varieties, and stages of tree health, which would help growers in Florida and other states where HLB also occurs.
The report also highlights the need to better understand the economic and sociological factors that impact decision-making and behaviors of growers, which influence the adoption of HLB management strategies. CRDF should create accessible databases to support sociological and economic modeling of citrus greening-related research outcomes and application projections.
The report recommends researchers communicate about the outcomes and evaluation of their efforts in a timely and systematic way. Additionally, current approaches to research prioritization and funding based within individual federal and state funding agencies have not led to development of a master plan for HLB research and subsequent management solutions. CRDF should work with other funding agencies to create an overarching advisory panel to develop a master plan for HLB research, communication, and management.
The study was sponsored by CRDF. The National Academies of Sciences, Engineering, and Medicine are private, nonprofit institutions that provide independent, objective analysis and advice to the nation to solve complex problems and inform public policy decisions related to science, technology, and medicine. The National Academies operate under an 1863 congressional charter to the National Academy of Sciences, signed by President Lincoln. For more information, visit http://national-academies.org. A roster follows.
To download full report: https://www.nap.edu/read/25026/chapter/1
hlb defprmed citrus