Posts Tagged: decline

Decline of Coast Live Oak Trees in Southern California Is Due to Fungus

We've been getting reports of coast live oak decline along the coast, well, here's one of the causes:

RIVERSIDE, Calif. — A fungus associated with the western oak bark beetle is causing a decline in coast live oak trees in Southern California by spreading “foamy bark canker disease.”

“We have found declining coast live oak trees throughout urban landscapes in Los Angeles, Orange, Riverside, Santa Barbara, Ventura and Monterey counties,” said Akif Eskalen, an assistant specialist in cooperative extension in the Department of Plant Pathology and Microbiology at the University of California, Riverside.

Eskalen recovered the fungal species, Geosmithia pallida, from tissues of infected coast live oak trees and performed pathogenicity tests on it in his laboratory at UC Riverside.  The tests showed that the fungus is pathogenic to coast live oak seedlings and produces symptoms of foamy canker.

The western oak bark beetle, which spreads the fungus, is a small beetle — about 2 millimeters long — that burrows through the bark of the coast live oak tree, excavating shallow tunnels under the bark across the grain of wood. Brown in color, this beetle is native to California. Female beetles lay their eggs in the tunnels.  It is not known at this time if the beetle infects trees other than coast live oak trees.

Symptoms of foamy bark canker disease include wet discoloration on the trunk and main branches of the infected coast live oak tree.  This discoloration surrounds the entry holes that the western oak bark beetle makes to burrow into the tree. Multiple holes can often be seen on an infected tree.

“When you peel back the outer bark of the infected area, you see bark (phloem) necrosis surrounding the entry hole,” Eskalen said. “As the disease advances, a reddish sap may be seen oozing from the entry hole, followed by a prolific foamy liquid.  This foamy liquid, the cause of which remains unknown, may run as far as two feet down the trunk.”

Eskalen explained that when the infection is at an advanced stage, the coast live oak tree dies.  Currently, no control methods are in place to control the fungus or the beetle.

If you suspect your coast live oak tree has the symptoms described above, please contact your local farm advisor, pest control advisor, county agricultural commissioner's office or Eskalen at akif.eskalen@ucr.edu.

 

foamy bark oak

Olive “Quick Decline” in southern Italy may be associated with pathogen common in California

 

Elizabeth Fichtner and Dani Lightle, Farm Advisors, UCCE Cooperative Extension                 

   The report of a new disease on olive in Italy, called “quick decline,” marks the first report of the bacterial pathogen, Xylella fastidiosa, in Europe. This pathogen is not new to the Americas and has been in California for over 100 years. It is perhaps best known as the cause of Pierce's Disease on grape, but also causes citrus variegated chlorosis, peach phony disease, alfalfa dwarf, and scorch on almond, oleander, and pecan. In response to scorch and dieback symptoms (Figure 1 A-C) on landscape and orchard plantings of olives in California, Dr. Rodrigo Krugner, an entomologist with the USDA ARS in Parlier, CA, established a research program to investigate the epidemiology of X. fastidiosa on California olives.

 

The Pathogen

X. fastidiosa is a gram-negative, xylem-limited bacterium affecting over 100 known plant hosts. The pathogen multiplies within the xylem and is thought to cause disease by interfering with water and nutrient transport. It is spread naturally from plant to plant by xylem-fluid feeding insects. The pathogen is difficult to culture (Figure 1D); consequently, prompt identification often relies on use of PCR techniques that detect pathogen DNA in plant tissues.

The pathogen may be grouped into subspecies based on host specificity. For example, X. fastidiosa subsp. fastidiosa causes Pierce's disease on grapevine as well as scorch on almond; however, the X. fastidiosa subsp. multiplex, causes disease on almond but not on grapevine.

Vectors associated with X. fastidiosa in California

X. fastidiosa is transmitted by xylem-fluid feeding insects, such as spittlebugs, froghoppers, and sharpshooters. While many of these insects may have the potential to transmit X. fastidiosa, there are four sharpshooter species in California that are recognized to have the greatest role in X. fastidiosa spread. Three of these sharpshooters are native to California and present throughout the state: red-headed sharpshooter, blue-green sharpshooter, and green sharpshooter. The last vector is the invasive glassy-winged sharpshooter (Figure 2A), which became established in southern California in 1990 and is responsible for the rapid spread of X. fastidiosa on grapevine.

Sharpshooters acquire X. fastidiosa when feeding on infected plant material. Once inside the vector's mouthparts, the bacterium multiplies rapidly and the insect is then capable of transmitting the bacterium for the remainder of its life (if it is an adult) or until it molts (if it is immature). Because sharpshooters are strong fliers and typically feed on multiple host plant species, X. fastidiosa may be spread to multiple hosts over the insects' lifetime.

‘Quick Decline' in Italy

In October 2013, X. fastidiosa was reported in the Puglia region of southern Italy, marking the first report of the pathogen in Europe. Characteristic symptoms included extensive leaf scorch and branch dieback, as well as discoloration of vasculature. Along with isolation of several putative fungal pathogens, presence of X. fastidiosa was confirmed by serological and PCR tests. Almond and oleander plants near the infected olives also tested positive for the pathogen. Scientists in Italy are currently surveying the area surrounding the outbreak and regulatory agencies have prohibited the movement of propagation materials from susceptible hosts out of the infected area. Additionally, researchers are working to determine the subspecies of X. fastidiosa associated with symptomatic olives and to obtain pure cultures of the pathogen for pathogenicity tests. Currently, the origin and strain(s) of X. fastidiosa introduced to Europe, as well as the insect species responsible for transmission, are unknown.

Association of X. fastidiosa with California olives

Leaf scorch and dieback symptoms have been observed in commercial olive orchards and landscape plantings (Figure 1 A and B) in California. Krugner's laboratory found that only 17% of the trees sampled tested positive for X. fastidiosa by PCR, with rates of pathogen detection higher in southern CA (39%) than in the Central Valley (2.5%). The pathogen was only successfully cultured from samples collected in southern California, suggesting that the pathogen population on olive is limited in the Central Valley. Reintroduction of the pathogen into multiple varieties of olive resulted in low levels of infection, and asymptomatic infections were common. Dr. Krugner's work also demonstrated that California strains of X. fastidiosa belong to the multiplex subspecies, which is pathogenic on almond, but not grapevine. Consequently, California olives are not considered a source of inoculum for Pierce's Disease on grapevine; however, olives may harbor insect vectors (Figure 2B) responsible for transmission of the bacterium to grapes or other crops.

What does the “'Quick Decline” in Italy mean for California olive growers?

Dr. Krugner's work demonstrated low levels of pathogen recovery from olives in the Central Valley and minimal association of the pathogen with disease upon reintroduction to healthy plants. Further studies, however, are necessary to determine a) the subspecies responsible for the ‘quick decline' in Italy, and b) the pathogenicity of isolates recovered from symptomatic plants in Italy. It is possible that pathogen strains recovered in Italy may be different, and more aggressive on olive, than strains endemic in California. California olive growers and landscape managers should report new incidences of extensive dieback or scorch on olives to farm advisors.

Acknowledgements

The authors thank Dr. Rodrigo Krugner for his critical review of this article and for providing photographs. Dr. Krugner's research was supported by the California Olive Committee and the USDA Agricultural Research Service.

Select References

Carlucci, A., Lops, F., Marchi, G., Mugnai, L., Surico, G. 2013. Has Xylella fastidiosa “chosen” olive trees to establish in the Mediterranean basin? Phytopathologia Mediterranea. 52:541-544.

Krugner, R., Sisterson, M.S., Chen, J., Stenger, D.C., Johnson, M.W. 2014. Evaluation of olive as a host of Xylella fastidiosa and associated sharpshooter vectors. Plant Disease. 98: in press.

Figures. The glassy-winged sharpshooter  Homalodisca vitripennis, is a known vector of Xylella fastidiosa. The winged sharpshooter can reproduce and overwinter on California olives.    In southern California landscapes, olives exhibited dieback  and leaf scorch. Six strains of Xylella fastidiosa subspecies multiplexwere isolated into pure culture from symptomatic olives trees in southern California. Photos: R. Krugner        Images of the pest and disease are in the attached files below.  Click on the link to see them.

 

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