Posts Tagged: biological fungicides
Year One of a Test of Biological Fungicides in Strawberries.
This is simply a summary of one year of biological fungicide work in strawberries in 2012-2013 and should not be understood as a recommendation to use any of these products. This investigation is will continue into 2013-2014 and will serve to confirm and adjust the work here.
Introduction: A number of biological fungicides registered for use in strawberries have not been thoroughly tested through empirical studies to give guidance to growers on their efficacy and use.
Materials and Methods:
Table 1 below is an outline of materials tested in 2012-2013.
The field involved in the study was organically farmed and had a tested infestation of Verticillium at the average level of 25 microsclerotia per treatment replicate. Grower standard was managed as per grower practice, that is to say normal irrigation, fertility and pest management practices were applied. This grower standard did not include any sort of biological fungicide either by dip or injection through the drip tape during the season.
Plot was replicated three times and each replicate consisted of at one bed of 180 feet in length.
Application of the materials took place as indicated in the table below:
Table 1. List of treatments.
Test Material |
Application/ Use |
Dazitol |
6.25 gal/A applied 3-5 days prior to planting |
Biotam + Serenade Soil |
5 lbs/A pre-plant application 3-5 days prior to planting followed by Serenade Soil @ 4 qt/A (10 days after planting, and then Serenade Soil @ 2 qt /A applied monthly after planting. Second application of Biotam 5 lbs/A and Serenade Soil @ 4 qt/A in February. |
Serenade Soil |
Serenade Soil @ 4 qt/A (10 days after planting, and then Serenade Soil @ 2 qt /A applied monthly after planting. |
Serenade Soil |
Serenade Soil @ 4 qt/A (10 days after planting, and then Serenade Soil @ 4 qt /A applied every 60 days after planting. |
Actinovate Rate 1 |
(1) 3 oz per 100 gal root dip at planting. (2) followed by 6 oz/A pre-plant in drip tape (3) followed by 3 oz/A every 30 days in drip tape
|
Actinovate Rate 2 |
(1) 3 oz per 100 gal root dip at planting. (2) followed by 6 oz/A pre-plant in drip tape (3) followed by 6 oz/A every 30 days in drip tape
|
SoilGuard |
Apply at planting as root dip or planting furrow drench at 5 lb/ A and again through drip tape every 4-6 weeks through harvest. |
Double Nickel 55 |
Apply at planting at 1 qt /A (1/2 lb/A powder) as root dip or planting furrow drench and again through drip tape every 4-6 weeks through harvest. |
Terra Clean 5.0 |
(1) 128 fl oz/100 gal drench (2) 2 gal/A drip applied at planting (3) 1 gal/A drip applied 10 days post plant and 28 days post plant (4) 1 gal/A drip applied 60, 90 and 120 days post plant |
Terra Clean 5.0 + Serenade Soil |
(1) 128 fl oz Terra Clean /100 gal drench + Serenade Soil @ 6 qt/ A soil drench (2) 2 gal/A Terra Clean drip applied at planting followed by SS @ 3 qt /A (3) 1 gal/A Terra Clean + 3 qt /A Serenade Soil drip applied 10 days post plant and 28 days post plant (4) 1 gal/A Terra Clean + Serenade Soil @ 3 qt/ A drip applied 60, 90 and 120 days post plant |
Tainio |
(1) Spectrum @ 50 g / A + Pepzyme C @ 12.5 oz/A 2- 3 days preplant (2) Biogenesis @ 1 lb/A+ Pepzyme C @ 12.5 oz/A as plant dip (3) Pepzyme C monthly through drip tape (4) Micro 5000 @ 2.66 oz/A at 2 leaf stage foliar |
Plant dips were made by suspending the requested rate of fungicide in approximately twenty gallons of water and submerging and soaking about a half a box of strawberry transplants (about 500 plants) thoroughly and then distributing to planting crews for transplant. Note that in the case of the two Actinovate treatments, plants were held for one night to prior to transplant, ostensibly to establish the organism on the plant roots.
Drip applications were made with a portable pump injecting each fungicide. Each application normally was preceded by filling the drip tape with clear water, injecting the mix and then further pumping in clear water to make sure the fungicide had moved well out of the drip tape.
Application dates:
Preplant application – 11/3/2012
Dazitol
Terra Clean 5.0
Terraclean 5.0 + Serenade Soil
Tainio Spectrum + Pepzyme C
Biotam
Root dip + one overnight hold – 11/7/2012 (planted November 8)
Actinovate rate 1
Actinovate rate 2
Root dip and immediate planting – 11/8/2012
Double Nickel 55
Soilguard
Biogenesis + Pepzyme C
10 days post – plant – 11/20/2012
Serenade 4 qt
Serenade 2 qt
Monthly applications (12/11/2012, 1/16/2013, 2/26/2013, 3/27/2013, 4/30/2013, and 6/13/2013)
Terra Clean 5.0
Terraclean 5.0 + Serenade Soil
Tainio Spectrum + Pepzyme C
Serenade 4 qt
Serendade 2 qt
Actinovate rate 1
Actinovate rate 2
Double Nickel 55
Biotam applied 2/26/2013; Serenade 4 qt per acre applied other dates in treatment
Soilguard
Several dying plants from different areas of the test plots were sampled in July to confirm that Verticillium was the cause of plant death.
As a gauge of plant vigor from each treatment, strawberry plant diameters were measured February 13, and April 13. Measurements were in centimeters and from twenty plants in each plot.
With the beginning of fruit ripening, fruit harvest was done weekly in each treatment replicate. On each pick date, fruit from each plot was weighed and counted.
Results:
|
Plant D cm 2/13/2013 |
Plant D cm 4/13/2013 |
Yield to 5/14/2013 |
April Total Yield g/plot |
Actinovate r1 |
14.05a |
16.25a |
3894.00a |
2175.00ab |
Actinovate r2 |
13.70ab |
16.42a |
3818.33a |
2428.00a |
Biotam |
11.47b |
15.77a |
2176.67b |
1377.00b |
Dazitol |
11.70b |
17.70a |
2505.67ab |
1568.00ab |
Double Nickel 55 |
12.65ab |
15.92a |
3084.00ab |
1637.00ab |
Serenade 4 qt +2 qt |
11.57b |
16.65a |
2853.00ab |
1471.67b |
Serenade 4 qt +4 qt |
12.42ab |
16.02a |
2621.00ab |
1459.67b |
Soilguard |
11.85b |
16.78a |
2993.33ab |
1652.33ab |
Tainio |
12.93ab |
16.95a |
3454.00ab |
2075.33ab |
Terraclean |
11.50b |
16.78a |
2284.00b |
1406.67b |
Terraclean + Serenade |
12.47ab |
17.11a |
2656.33ab |
1559.67ab |
Untreated grower standard |
11.70b |
17.90a |
2145.00b |
1189.00b |
Means followed by same letter do not significantly differ (P=.05, Student-Newman-Keuls)
|
May Total Yield g/plot |
June Total Yield g/plot |
July Total Yield g/plot |
Aug Total Yield g/plot |
Total Yield g/plot |
Actinovate r1 |
3741.67a |
2791.00a |
1902.33a |
140.64a |
16820.67a |
Actinovate r2 |
3069.67a |
3237.67a |
2812.33a |
149.35a |
17201.34a |
Biotam |
2254.00a |
3251.33a |
3402.00a |
503.14a |
14468.33a |
Dazitol |
2514.67a |
3075.33a |
3049.33a |
269.16a |
14625.00a |
Double Nickel 55 |
3038.67a |
2837.67a |
2573.67a |
203.64a |
15052.67a |
Serenade 4 qt +2 qt |
3244.67a |
3287.00a |
3623.67a |
371.49a |
16887.67a |
Serenade 4 qt +4 qt |
2959.67a |
3617.67a |
3236.33a |
362.02a |
16157.00a |
Soilguard |
3212.33a |
3331.67a |
2887.00a |
359.45a |
16352.33a |
Tainio |
3409.00a |
3687.33a |
3190.33a |
170.27a |
18145.00a |
Terraclean |
2496.67a |
2997.00a |
2750.00a |
125.01a |
13681.33a |
Terraclean + Serenade |
2544.00a |
2810.00a |
2230.00a |
191.78a |
13454.33a |
Untreated grower standard |
2812.00a |
4019.33a |
3800.00a |
306.15a |
16150.33a |
Means followed by same letter do not significantly differ (P=.05, Student-Newman-Keuls)
Plant diameters measured on February 13, 2013, were significantly larger in the low rate of Actinovate than the other treatments with the exception of the high rate of Actinovate, Tainio and Double Nickel 55.
Both rates of Actinovate realized higher fruit yield than all other treatments except for the Dazitol, in cumulative fruit yield, which included six weekly harvests, up to May 14, 2013. No further differences were realized between May and August.
As noted above, this field has a very high infestation of Verticillium and all plots began to experience pronounced plant dieback in June, with some 60-80% remaining alive or declining and by end of July, nearly all plants in all replicate plots had died. There was a trend for plants which had produced larger amounts of fruit in April and May to experience lesser fruit production lesser vigor and earlier dieback as the season progressed.
Conclusion
The results of this trial are encouraging. In the early part of the season up through the middle of May, several treatments had significantly higher amounts of fruits harvested than the grower standard. Nonetheless, none of the treatments provided sufficient protection to the plants to prevent an almost complete die out of the plants by August, effectively ending the season.