Organic strawberry yields boosted by technique refined through UCCE research

There is a stark difference in plant vigor between an ASD-treated plot (left) and a standard untreated plot in an organic field infected with charcoal rot. Photo by Joji Muramoto

Anaerobic soil disinfestation helps suppress weeds, disease without fumigants

Troubled by puny plants, low yields and persistent mite problems, third-generation Southern California strawberry grower Glen Hasegawa was ready to give up on his transition from conventional to organic 12 years ago.

“I've always liked a challenge – but it turned out to be more of a challenge than I thought it would be!” he said.

But then, with the help of scientists including Oleg Daugovish, UC Cooperative Extension strawberry and vegetable crop advisor in Ventura County, Hasegawa tried a technique called anaerobic soil disinfestation (ASD). When applied correctly, the multi-step ASD process creates a soil environment that suppresses pathogens and weeds and makes for healthier, more robust crop growth.

“Back in the day, it was really hard to get the plant growing vigorously in organic,” said Hasegawa, owner of Faria Farms in Oxnard. “So we started using the ASD and then you could definitely see that the plant had more vigor and you could grow a bigger, better plant using it.”

Seeing that he could produce yields “in the neighborhood” of those grown in conventional strawberry fields fumigated with synthetic fumigants, Hasegawa was able to expand his original 10 acres of organic strawberries to 50 acres.

“I guess you could say I'm kind of a convert,” he said, noting that he now applies ASD to all his acreage each year in late spring.

Joji Muramoto, UC Cooperative Extension specialist in organic production based at UC Santa Cruz, has been experimenting with ASD since it was first brought to the U.S. from the Netherlands and Japan in the early 2000s. Carol Shennan, a professor in the Department of Environmental Studies at UCSC, and Muramoto were among the first to try the technique in California. They found that ASD successfully controlled an outbreak of Verticillium wilt – caused by the pathogen Verticillium dahliae – at UCSC's small organic farm in 2002.

Since then, Shennan, Muramoto, Daugovish and their colleagues have seen encouraging results at 10 trial sites across the state.

“We demonstrated that ASD can provide comparable yields with fumigants, in side-by-side replicated trials,” Muramoto said.

The strawberry plants in the ASD-treated plot (left) are more robust than those growing in untreated soil. A plastic mulch (typically opaque is used by growers, but clear was used here for research purposes) is crucial as part of the ASD process to improve plant productivity and control weeds. Photo by Oleg Daugovish

ASD promotes host of beneficial changes to soil ecosystem

ASD comprises three basic steps: incorporating a carbon source that is easily digestible by microbes in the soil (traditionally, rice bran has been used), further encouraging fermentation by covering the soil with plastic to limit oxygen supply, and finally adding water through drip irrigation to initiate the “anaerobic” decomposition of the carbon source and maintain the three-week “cooking” process.

The resulting cascade of chemical, microbiological and physical changes to the soil creates an ecosystem that is both conducive to strawberry growth – and inhospitable to pathogens and weeds.

“It's not like a pesticide where you have a mode of action, and thus resulting in ‘A' and ‘B' for you,” Daugovish explained. “There's a sort of cocktail of events that happens in the soil; they all happen interconnectedly.”

Compared to similar fields that did not undergo the process, ASD-applied organic strawberry fields across California have seen yields increase by 60% to 70% – and even doubling in some cases, according to Daugovish.

The UCCE advisor also shared the story of a longtime grower in Ventura County, who came to him with fields in “miserable” condition; they were plagued by one of the world's worst weeds, yellow nutsedge, and infected with charcoal rot, a disease caused by the fungus Macrophomina phaseolina. But after applying rice bran and following the ASD recipe, the grower saw phenomenal results.

“The only complaint he said to me was, ‘Now I have too many berries – we have to have more pickers to pick the berries!'” Daugovish recalled.

Via researchers' meetings, online resources, on-farm demonstration trials and word of mouth from peers, use of ASD by California strawberry growers has grown significantly during the past two decades. Tracking the purchase of rice bran, Muramoto estimated that about 2,500 acres were treated by the ASD-related practices in 2023 – covering roughly half of the 5,200 total acres of organic strawberries in California.

Muramoto directly links the growth of California organic strawberry production – which now comprises about 13% of total strawberry acreage in the state – with the increasing adoption of ASD.

“If you remove the acreage with the applied rice bran over the last 10 years or so, organic strawberry acreage is just flat,” he said.

Applying rice bran provides the easily digestible carbon source that soil microbes feed on. Once covered by plastic mulch, anaerobic conditions develop and microorganisms that prefer such an environment thrive, while pathogenic organisms and germinating weeds are suppressed. Before planting, holes are cut in the plastic to let oxygen back into the soil and encourage the growth of the strawberry plants. Photo by Oleg Daugovish

Within the last decade, acreage of organic strawberries with ASD-related practices increased by 1,640 acres, which is a boon for air quality, human health and long-term soil vitality. According to Muramoto's calculations, that increase in organic acreage translates to a reduction of about 465,000 pounds of fumigant active ingredients that would have been used in growing conventional strawberries.

“There are hundreds of reports of acute illnesses related to fumigation in the record, so it's very important to find alternatives to fumigants,” said Muramoto, citing California Department of Pesticide Regulation documents.

Research continues to make ASD more economical, effective

The popularity of ASD has come at a price, however, for organic strawberry growers.

“There's more organic out there, and I think most of the organic guys are using it, so there's more demand on the rice bran; the price has been steadily going up every year, like everything else,” said Hasegawa, adding that he has been trying to decrease the amount of carbon while maintaining ASD's efficacy.

On top of greater demand from other growers and from beef cattle and dairy producers (who use rice bran as feed), the price also has increased due to higher costs in transporting the material across the state from the Sacramento Valley. So Daugovish and his colleagues – including Peter Henry, a U.S. Department of Agriculture plant pathologist – have been searching for a cheaper alternative.

“We all want an inexpensive, locally available, reliable, easy to use and functional carbon source, which sounds like a big wish list,” Daugovish said.

Carbon sources such as bark, wood chips, or compost are ineffective, as the crucial ASD microorganisms are choosy about their food.

In a field of 'Victor' strawberries, researchers saw a 41% increase in yield using ASD with midds, in comparison to untreated soil. Photo by Oleg Daugovish

“Microbes are just like cows; you can't feed them straight wood; they get pretty angry,” Daugovish explained. “And if you feed them something with too much nitrogen, they can't digest it – they get the runs. Microbes are the same way – you have to have the right proportion of stuff so they feel comfortable doing what they're doing.”

In search of an ideal replacement, researchers tried and ruled out grass clippings, onion waste, glycerin and coffee grounds. Finally, they pivoted to a material with properties very similar to rice bran: wheat bran, in the form of wheat middlings (also called midds, a byproduct of flour milling) and dried distillers' grain (DDG, a byproduct of ethanol extraction).

After field experiments in Santa Paula, the UC and USDA researchers found that midds and DDG were just as effective at controlling soilborne pathogens and weeds as rice brain – but at 25% to 30% less cost. Their results were published last year in the journal Agronomy.

“Not surprisingly, the wheat bran has worked almost exactly the same as rice bran,” Daugovish said.

He and Muramoto are now conducting trials with wheat bran at commercial fields, and the initial results are promising. Daugovish said the grower at one site in Ventura County has seen a 90% reduction in Macrophomina phaseolina, the causal pathogen of charcoal rot, in the soil – and an 80% to 90% drop in yellow nutsedge germination. They are waiting for final yield numbers after the coming summer.

While ASD has been beneficial to organic productivity and soil health, both Daugovish and Muramoto acknowledged specific limitations in suppressing the “big three” strawberry diseases: Verticillium wilt, Fusarium wilt and charcoal rot. In coastal areas with cooler soil temperatures, for example, ASD can actually exacerbate the latter two diseases, as the fungal pathogens feed on the rice bran.

“We know it works at warmer temperatures, but, practically, it's hard to do in coastal California,” Muramoto said. “It would be nice if we can find a way to suppress Fusarium wilt at a lower temperature, but we don't have it right now.”

That's why researchers emphasize that ASD is not a “silver bullet.” It's just one tool in the organic toolbox, which includes careful crop rotation, disease-resistant strawberry varieties and better diagnostic tests that help growers pinpoint outbreaks and make the application of various methods more targeted and more efficient.

And scientists will continue to optimize ASD to make it more effective and economical for growers in the different strawberry regions of California – from the Central Coast to the Oxnard Plain.

“We know it can work really well; it's just finding the most sustainable way to do this in our region,” Daugovish said. “We've got to just have an open mind and keep trying.”

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